| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072728 |
| Chromosome: | chromosome 10 |
| Location: | 4904522 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454734 | TIC21,PIC1 | (1 of 1) PTHR21649//PTHR21649:SF13//PTHR34548 - CHLOROPHYLL A/B BINDING PROTEIN // LHCA2 PROTEIN // FAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGATGTGGAGATGGGTACGTACCCCG |
| Internal bar code: | GGCCAGCGCTGGGCGCGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 99.906 |
| LEAP-Seq n confirming: | 1063 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGATCAGCTCCTACCC |
| Suggested primer 2: | CACACTCGCTCCACACTAGC |