| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072771 |
| Chromosome: | chromosome 1 |
| Location: | 3160065 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGATCTGGCGCAGGTAGCGCAGGATGT |
| Internal bar code: | CAAAGCTGTAAGCACTCGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 99.6711 |
| LEAP-Seq n confirming: | 2727 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACACAAGTGAGCCCCAGG |
| Suggested primer 2: | CTTGTCCACTCGGTCGAACT |