| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072853 |
| Chromosome: | chromosome 9 |
| Location: | 3131006 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386912 | DGTT2 | Diacylglycerol acyltransferase, DGAT Type 2; (1 of 4) K14457 - 2-acylglycerol O-acyltransferase 2 (MOGAT2, MGAT2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTTGTGGCCGAGCCCATTTGCAGGTC |
| Internal bar code: | CAGTCGCCGTGGCCTCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 97.8022 |
| LEAP-Seq n confirming: | 178 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCAGTATGCGCTATGG |
| Suggested primer 2: | GGCATGCGAGGTAAGTTCAT |