| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072867 |
| Chromosome: | chromosome 10 |
| Location: | 4502921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451950 | AAT1 | (1 of 1) 2.6.1.4 - Glycine transaminase / Glycine aminotransferase; Alanine aminotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATCTCCACGTAGCCGCCGCGCAGGCCG |
| Internal bar code: | GTCTGCCAGGGAGTATAGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.4186 |
| LEAP-Seq n confirming: | 7182 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTGAGCAAGGAGAACC |
| Suggested primer 2: | CCTCCAGAAGTTTGAGGCAG |