| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072901 |
| Chromosome: | chromosome 17 |
| Location: | 5178807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735550 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGTGCATAAGCTAATGTGGTACATAGT |
| Internal bar code: | ACACAGGAGTCCCATTCGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 97.2125 |
| LEAP-Seq n confirming: | 4987 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGGTGTATCCGGGGTTG |
| Suggested primer 2: | TGGTGAGTTGGGAAGTGTGA |