| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072929 |
| Chromosome: | chromosome 7 |
| Location: | 2894193 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332500 | AXT2 | (1 of 3) PTHR10994:SF69 - PROLINE-RICH PROTEIN 18; Arabinose chain extension enzyme for HRGP 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGCCCTAAAACTCTATCGACCGCCGA |
| Internal bar code: | CGCGCGTTTACTTGTGGCTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.7262 |
| LEAP-Seq n confirming: | 6557 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTATGATGAGCTTGGCG |
| Suggested primer 2: | CACAACACTGATCACCGACC |