| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072935 |
| Chromosome: | chromosome 1 |
| Location: | 4701364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032400 | (1 of 1) PF05118 - Aspartyl/Asparaginyl beta-hydroxylase (Asp_Arg_Hydrox) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATGGCGGAGCTCAAGCGCGCCGGTGT |
| Internal bar code: | TCGCCTATCCCGTCCGTGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1312 |
| LEAP-Seq percent confirming: | 99.5957 |
| LEAP-Seq n confirming: | 1478 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGTAGCATGCAGACAAA |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |