| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072938 |
| Chromosome: | chromosome 17 |
| Location: | 3346939 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGAGACCGAGAGCCGCAGCACAGCGGG |
| Internal bar code: | ATGCTCACCCCGTTTTAACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.2713 |
| LEAP-Seq n confirming: | 3678 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGCTCTAGGCGACATT |
| Suggested primer 2: | CCGCTAGCTTGAGTAATCCG |