Insertion junction: LMJ.RY0402.072938_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.072938
Chromosome:chromosome 17
Location:3346947
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g723500 (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCCCCTGTCCCCTGCTCACCCTGTGTT
Internal bar code:GCCCCATCATCCAGTGGTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:95.2618
LEAP-Seq n confirming:382
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTAGCTTGAGTAATCCG
Suggested primer 2:AGCATGCTCTAGGCGACATT