| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.072951 |
| Chromosome: | chromosome 16 |
| Location: | 2449103 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660601 | (1 of 1) IPR000772//IPR003609 - Ricin B, lectin domain // PAN/Apple domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGTAAATAGGGCCAGCGCATGTAAGG |
| Internal bar code: | TGGTCAATAGCCGTCCATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 99.5987 |
| LEAP-Seq n confirming: | 1489 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAGACGCTGTGACCTGA |
| Suggested primer 2: | TTGTTACACCCATCCCACCT |