| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072967 |
| Chromosome: | chromosome 6 |
| Location: | 3853142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278173 | OPR59 | OctotricoPeptide Repeat protein 59; (1 of 1) K08515 - vesicle-associated membrane protein 7 (VAMP7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCACGGCGGCTTTAGTGGCCGCGGCAG |
| Internal bar code: | GAGGGAAATCATGCCCAAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGAGTCTCCAAGTGTC |
| Suggested primer 2: | TGCTGTTACTATTGCTGCCG |