| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.072982 |
| Chromosome: | chromosome 7 |
| Location: | 384344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314800 | Coiled-Coil Protein; (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGCCTGTCGCTCCAGTTTGGTGGCGA |
| Internal bar code: | TTATTACCTAGTACCGGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 96.1938 |
| LEAP-Seq n confirming: | 834 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGGGCGTGTGTTAAG |
| Suggested primer 2: | CATGCGATGCAGAATTCAAG |