| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072989 |
| Chromosome: | chromosome 17 |
| Location: | 5029343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734757 | TET5 | (1 of 12) PF12851 - Oxygenase domain of the 2OGFeDO superfamily (Tet_JBP); {"non-canonical TET-dioxygenase, putative 5-methylcytosine-modifying enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATGAAGGTGTGCAGAACTCGCAACGTT |
| Internal bar code: | GGGGGGTGGCCCCCGGTGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 98.9992 |
| LEAP-Seq n confirming: | 1187 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCGGGTGGGTAGTAGT |
| Suggested primer 2: | ATCACCCAACCAGTTTGCTC |