| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073003 |
| Chromosome: | chromosome 4 |
| Location: | 364213 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217912 | (1 of 1) PTHR13035//PTHR13035:SF0 - UNCHARACTERIZED // PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCAGGTGCGGTGCCGTACTGCACCTT |
| Internal bar code: | CTCGCGAAAGTAATCTGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 98.6038 |
| LEAP-Seq n confirming: | 6921 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGATACCCATCTGGCAGC |
| Suggested primer 2: | GGCCTGTACCTCACCCAGTA |