| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073009 |
| Chromosome: | chromosome 11 |
| Location: | 509621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGCGATCCACGTGCAAGCGCTCCGGC |
| Internal bar code: | CTGCCGTGGCTCGTAGAACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 91.635 |
| LEAP-Seq n confirming: | 482 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCATGATAGGGGTACTGC |
| Suggested primer 2: | GCAGCGATTTGCAACAGTAA |