Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.073035 |
Chromosome: | chromosome_1 |
Location: | 2613476 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g015200 | PDE4 | 3\',5\'-cyclic-nucleotide phosphodiesterase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGGCACGAGGCTTGTTATGGTCTAGCGA |
Internal bar code: | TTACGAAGTACAAGAAAGTCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 89 |
LEAP-Seq percent confirming: | 99.5081 |
LEAP-Seq n confirming: | 2630 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCGACACACATGCCAGTAT |
Suggested primer 2: | CTCCGTCATGCATGTACACC |