Insertion junction: LMJ.RY0402.073035_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.073035
Chromosome:chromosome 11
Location:2478138
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACCGTGAAGGCTGGCATTGAGGCTGTG
Internal bar code:AGCCACTCGAGATAGCGGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:34.2508
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:215
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGCTAGATGAGGAGCTG
Suggested primer 2:CTGCACGTATCGCAGAATGT