Insertion junction: LMJ.RY0402.073041_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.073041
Chromosome:chromosome 9
Location:7511192
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g414000 VTE8,HPT1 (1 of 1) 2.5.1.116 - Homogentisate geranylgeranyltransferase / HGGT; homogentisate phytyltransferase/ prenyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCAGCCCTTTCACTGAGAGTCTCGCGC
Internal bar code:AGTTCACGCGACGTTGCAAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:76.25
LEAP-Seq n confirming:427
LEAP-Seq n nonconfirming:133
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCCTCCTCATTACGAATC
Suggested primer 2:CCGTACTGTTCTCGGGTCAT