| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073051 |
| Chromosome: | chromosome_5 |
| Location: | 3034573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g238650 | PHC5 | Cell wall protein pherophorin-C5 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTGACGAGATGGCGTAATAACACCAGGT |
| Internal bar code: | TCCGCGGTGGAGGAACCGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGTGATAGTGCTCGTCA |
| Suggested primer 2: | TTGTCTCAGAAGATGTGGCG |