Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.073051 |
Chromosome: | chromosome_8 |
Location: | 2557428 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g372500 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATCTTCGCCTTTCTGCATACCGTACATT |
Internal bar code: | GGGGTTTGAGAGTCCTATCACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 822 |
LEAP-Seq percent confirming: | 92.589 |
LEAP-Seq n confirming: | 3823 |
LEAP-Seq n nonconfirming: | 306 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTATGGCTGCACAAGAAG |
Suggested primer 2: | TACCAGCCCAAAGTAAACCG |