Insertion junction: LMJ.RY0402.073053_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073053
Chromosome:chromosome 4
Location:3677050
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g228900 PPP19 (1 of 1) K17618 - ubiquitin-like domain-containing CTD phosphatase 1 [EC:3.1.3.16] (UBLCP1); Phosphoprotein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTCGCGACACGCCGACCAAGCGCGTTG
Internal bar code:ATTCGTGGACAAAACCGGAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:97.2973
LEAP-Seq n confirming:540
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCCAAAGCAATACCGTC
Suggested primer 2:CGGTGTGTGTGTGTGTGTGT