Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.073054 |
Chromosome: | chromosome 12 |
Location: | 3508336 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGTTGAAACACTCGGTTGACAAACAA |
Internal bar code: | AATGTCGGCTTTGCCGGGGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 804 |
LEAP-Seq percent confirming: | 97.1331 |
LEAP-Seq n confirming: | 1999 |
LEAP-Seq n nonconfirming: | 59 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTGTTTGACACGGCTCAT |
Suggested primer 2: | GGATGCCATACCGTAGTGCT |