| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073065 |
| Chromosome: | chromosome 3 |
| Location: | 4032094 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172250 | (1 of 1) PTHR11649:SF5 - GTP-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCCGACATGCCGCGCCATCATGTTGCC |
| Internal bar code: | AGACGGGTTTGGAAGAAATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.1316 |
| LEAP-Seq n confirming: | 1484 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCGGAGTCAACAACAG |
| Suggested primer 2: | TTACGAACCGGCCTTGTTAC |