Insertion junction: LMJ.RY0402.073079_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073079
Chromosome:chromosome 3
Location:925930
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g146807 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGATCGTTTGTGCCCGCGGTGCCGAAAC
Internal bar code:ACCCCTTGTAAAGTCGGCACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:44.0266
LEAP-Seq n confirming:1990
LEAP-Seq n nonconfirming:2530
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGTCGCACGAATGTATG
Suggested primer 2:GCAGGTGAAGGTGAGAGGTC