Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.073122 |
Chromosome: | chromosome 16 |
Location: | 1149746 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g650350 | (1 of 1) PF03457 - Helicase associated domain (HA) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCCATCTTGCGTTTGGTGCCGTTGCCC |
Internal bar code: | CAAAAGACTAACAGAAGTGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 546 |
LEAP-Seq percent confirming: | 99.2775 |
LEAP-Seq n confirming: | 2061 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCGTTGATGTGGAGAAAGG |
Suggested primer 2: | GGTGGTGTGGTGTCAGTTTG |