Insertion junction: LMJ.RY0402.073146_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073146
Chromosome:chromosome 4
Location:385039
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217915 NAR1C Formate/nitrite transporter; (1 of 1) IPR000292//IPR023271 - Formate/nitrite transporter // Aquaporin-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATCCGCGCCAACCACCTGTTCGGTTAGT
Internal bar code:CGCGAATGTCGATGGCGAGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:9
LEAP-Seq percent confirming:0.697527
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:3132
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTGCAAATGATGCCCTGT
Suggested primer 2:AACCGACATGACTAGCCCAC