| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073150 |
| Chromosome: | chromosome 12 |
| Location: | 70151 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483800 | CPY | Serine carboxypeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGATGCTGATGCTACTGGTGTAGCTG |
| Internal bar code: | GCGAGATACGTCAACCTTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 71.7241 |
| LEAP-Seq n confirming: | 312 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGAGGTCACAGCTCTCC |
| Suggested primer 2: | CAGACAAGCGTTCTTCCTCC |