Insertion junction: LMJ.RY0402.073197_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073197
Chromosome:chromosome 4
Location:1764228
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g212200 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCCAAAGAGACGGGCTACCGGCACACA
Internal bar code:GGCTCGTGGACCCCAAACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:88.7232
LEAP-Seq n confirming:1487
LEAP-Seq n nonconfirming:189
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCATTGCACAACACTTG
Suggested primer 2:CACTATGGTGGTACGATGCG