| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073226 |
| Chromosome: | chromosome 12 |
| Location: | 648296 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492152 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATTAATTGCCTCCGCTTCCATACAT |
| Internal bar code: | CTGCCTGGTTGACCCGTGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 99.0189 |
| LEAP-Seq n confirming: | 12010 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCGTCTACCTCATCTCC |
| Suggested primer 2: | TAAGGGTGGGAGGGCTAAGT |