| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073231 |
| Chromosome: | chromosome 3 |
| Location: | 4107943 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGCATACGGCTCTGTGGGAAATAAGC |
| Internal bar code: | TAGACGGGCTCTAGGCGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 76.0539 |
| LEAP-Seq n confirming: | 6711 |
| LEAP-Seq n nonconfirming: | 2113 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTGTGCAAAGGTGTGG |
| Suggested primer 2: | TGAAGCCCTTTCCAAATCAC |