Insertion junction: LMJ.RY0402.073245_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073245
Chromosome:chromosome 11
Location:2869249
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g477750 (1 of 1) IPR016201 - Plexin-like fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTGGGCGAATGCTGCTCGCGGCGCAA
Internal bar code:GACACAACGTCCCGTCTTTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:833
LEAP-Seq percent confirming:99.4647
LEAP-Seq n confirming:929
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTATGTTCCGTTGGTGCGTA
Suggested primer 2:CGCACCCTCTTAACTCTTCG