| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073248 |
| Chromosome: | chromosome 12 |
| Location: | 2637474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504400 | (1 of 1) 2.3.1.22 - 2-acylglycerol O-acyltransferase / Monoglyceride acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGATGCCGGATGGCGTGTGTGTGCAGT |
| Internal bar code: | TTGACGACTGCCGTTGTATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 82.0 |
| LEAP-Seq n confirming: | 656 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGTGTCTACCACGAAG |
| Suggested primer 2: | TCACCACTCCCACTCACGTA |