| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073250 |
| Chromosome: | chromosome 17 |
| Location: | 4530555 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732650 | (1 of 1) K17601 - WD repeat-containing protein 81 (WDR81) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCAGCAGTATGAAGCGCAGGTGGCTGT |
| Internal bar code: | GATTATTGGCCGGCTCCGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 94.5011 |
| LEAP-Seq n confirming: | 1667 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCGGTTACCCAAGGGAC |
| Suggested primer 2: | ACACCGCAATCCTACTGGAC |