| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073256 |
| Chromosome: | chromosome 5 |
| Location: | 1190581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245500 | FAP175,ANK24 | (1 of 1) PTHR24133//PTHR24133:SF21 - FAMILY NOT NAMED // ANKYRIN REPEAT-CONTAINING PROTEIN; Ankyrin Repeat Flagellar Associated Protein 175 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGCTGCTGGGTCTGTGCGCAATGAAGT |
| Internal bar code: | ATCACGCAGGGAGAGTGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 98.7274 |
| LEAP-Seq n confirming: | 3336 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACCAAACACACCCGGA |
| Suggested primer 2: | CACGACCATGGCTGTGTTAC |