Insertion junction: LMJ.RY0402.073257_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.073257
Chromosome:chromosome 3
Location:9046273
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g210513 (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCAGTCCAAGCTATACCATACCCATGC
Internal bar code:GCGGACTGCACGCGCGGTCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:412
LEAP-Seq percent confirming:99.3831
LEAP-Seq n confirming:1450
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACTCTCCCCGACTCTTG
Suggested primer 2:GGGCTCTGCTTTACGATCTG