Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.073257 |
Chromosome: | chromosome 3 |
Location: | 9046273 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCAGTCCAAGCTATACCATACCCATGC |
Internal bar code: | GCGGACTGCACGCGCGGTCCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 412 |
LEAP-Seq percent confirming: | 99.3831 |
LEAP-Seq n confirming: | 1450 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCACTCTCCCCGACTCTTG |
Suggested primer 2: | GGGCTCTGCTTTACGATCTG |