| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073259 |
| Chromosome: | chromosome 7 |
| Location: | 2285114 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327750 | TRP3 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCTTGCCTGGCGTGTGTGTGCGTGTG |
| Internal bar code: | TAGGGAGTGGAATTTGCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.6533 |
| LEAP-Seq n confirming: | 7761 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCATCTTTCCACCCTGAA |
| Suggested primer 2: | CTGAAGGTTAAGCAGCAGGG |