| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073261 |
| Chromosome: | chromosome 9 |
| Location: | 7190526 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412175 | (1 of 2) 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase / HOHPDA hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGCGCCGCCGCCGGCTGTAGCAGCGC |
| Internal bar code: | TCCCGCCATAAGTCGCGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 72.3263 |
| LEAP-Seq n confirming: | 1312 |
| LEAP-Seq n nonconfirming: | 502 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAATCGAATGCGCTCAA |
| Suggested primer 2: | ACTTGACTGCTCGCCGTACT |