| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073265 |
| Chromosome: | chromosome 15 |
| Location: | 177908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635100 | (1 of 2) IPR000104//IPR010402 - Antifreeze protein, type I // CCT domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTCGCCGTACCCCTCATCCTGACCTTC |
| Internal bar code: | AGTCGTCGGCGGGTTGTATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 66.4311 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATATCAGGGCGTGTGGTA |
| Suggested primer 2: | CATGAGGTGTACATGGCGAC |