| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073272 |
| Chromosome: | chromosome 14 |
| Location: | 3683430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631600 | (1 of 1) 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCATCAGCTCCCCCAACCCCCAGTGGC |
| Internal bar code: | CCTGCGGAAGGGAGTCCGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 98.3891 |
| LEAP-Seq n confirming: | 3909 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAACCGTGGGTTATATGG |
| Suggested primer 2: | GCTAGGACGACTTTGGTTCG |