Insertion junction: LMJ.RY0402.073293_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.073293
Chromosome:chromosome 5
Location:164607
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g234400 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTGCGCCATCTCAATGCTCACACTCC
Internal bar code:TGTCCGTCAAATGTCCGTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:130
LEAP-Seq percent confirming:51.0609
LEAP-Seq n confirming:1468
LEAP-Seq n nonconfirming:1407
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGACACCGTGATTGAAG
Suggested primer 2:TAGAGCAGATGGGTGTGCTG