| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073301 |
| Chromosome: | chromosome 6 |
| Location: | 2992161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCCACCGCCGCCGCACCTGCGCCCTGC |
| Internal bar code: | GTAGGATCTCCGGGGCAAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 83.6066 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCAAGCCCAAGGTAAG |
| Suggested primer 2: | GTGCTGTACAATGTGGGACG |