Insertion junction: LMJ.RY0402.073303_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073303
Chromosome:chromosome 6
Location:2790190
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g271150 FAP74,C1d-HC3 (1 of 6) PF14874 - Flagellar-associated PapD-like (PapD-like); Flagellar Associated Protein 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACGTGTCCGCCAGCAGCTGTAGGCTGGT
Internal bar code:GAGCAATCGGTAAGGTTTGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:791
LEAP-Seq percent confirming:98.4082
LEAP-Seq n confirming:1051
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGCTGTGGACTTGAGCA
Suggested primer 2:TACCGTACATAACGCGTGGA