Insertion junction: LMJ.RY0402.073303_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.073303
Chromosome:chromosome 6
Location:2790197
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g271150 FAP74,C1d-HC3 (1 of 6) PF14874 - Flagellar-associated PapD-like (PapD-like); Flagellar Associated Protein 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGTCGGATGGGGTTGGTTGTTGATATC
Internal bar code:GCTAGGGCCATAATACAGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:92.5319
LEAP-Seq n confirming:1016
LEAP-Seq n nonconfirming:82
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGCTGTGGACTTGAGCA
Suggested primer 2:TACCGTACATAACGCGTGGA