| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073319 |
| Chromosome: | chromosome_10 |
| Location: | 5606471 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g460050 | FLU | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAATGAGCTGGCCGCTATGGTCCCATTAGG |
| Internal bar code: | TCCCAACGGATTCCGCTTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 84.6695 |
| LEAP-Seq n confirming: | 602 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTACACCTAACGTCCAT |
| Suggested primer 2: | GCTGCATACCTCGTCTGTGA |