Insertion junction: LMJ.RY0402.073333_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073333
Chromosome:chromosome 10
Location:4847758
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g454250 FKB13,FKB16-9,FKB16I Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PTHR10516:SF308 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP17-2, CHLOROPLASTIC-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTTGAAGGTCTGGCAGCGCAGCAGCGAC
Internal bar code:CCAGCCGCCTTGTCAGCGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:175
LEAP-Seq percent confirming:70.3002
LEAP-Seq n confirming:445
LEAP-Seq n nonconfirming:188
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACAATCACAGCCCCTCAAC
Suggested primer 2:GAGTGCTTTGTCAGGGCTTC