| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073355 |
| Chromosome: | chromosome 16 |
| Location: | 2891542 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGAGCTGCCGGACCTGCTGCTGCCGC |
| Internal bar code: | GATTATCATGAGTGGTGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 81.9202 |
| LEAP-Seq n confirming: | 657 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGAGAGGAGAACACGAG |
| Suggested primer 2: | TTTGGGATATCATGGACCGT |