| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073405 |
| Chromosome: | chromosome 9 |
| Location: | 1630588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397200 | CCT6 | T-complex protein 1, zeta subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCCAGCGCCTCGACGCGAGTTAGCTGT |
| Internal bar code: | GATACTCGGGCATCTGCAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 21.7241 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 227 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCCTGTCGATGTCTCT |
| Suggested primer 2: | CGTCAAGAGGGATGGTTGAT |