Insertion junction: LMJ.RY0402.073540_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.073540
Chromosome:chromosome 7
Location:2402665
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g328600 (1 of 1) K11804 - WD repeat-containing protein 42A (WDR42A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCTTCAACCGTCTCACGCGCTTCAATC
Internal bar code:ATATGGTGAGGACGGGGTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:74
LEAP-Seq percent confirming:99.5902
LEAP-Seq n confirming:243
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCAACCAGAAGAAGACA
Suggested primer 2:GATCCACGACCTCCACCTTA