Insertion junction: LMJ.RY0402.073546_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.073546
Chromosome:chromosome 3
Location:2630850
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g160900 (1 of 1) PF07714//PF12796//PF13857 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACGACAACGATGCAATGCTGCGGCTAC
Internal bar code:CGCAGTGACGCGACACTGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:52.5362
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:131
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGAATGGTTGTGCTGCT
Suggested primer 2:ACCACACAGGACGCACATAA