| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073564 |
| Chromosome: | scaffold 19 |
| Location: | 142994 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750897 | (1 of 1) 3.5.4.12 - dCMP deaminase / Deoxycytidylate deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTGTTTCATACGTTGATCATATCAAA |
| Internal bar code: | GTTACTTTAGCCACACATCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 98.8705 |
| LEAP-Seq n confirming: | 6915 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGGAAACCCCAATCAT |
| Suggested primer 2: | TTTCCATCTCCGGATGAGAC |